pgl 3-basic vector Search Results


jetpei  (Polyplus-transfection SA)
95
Polyplus-transfection SA jetpei
Jetpei, supplied by Polyplus-transfection SA, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/jetpei/product/Polyplus-transfection SA
Average 95 stars, based on 1 article reviews
jetpei - by Bioz Stars, 2026-04
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inserted into the pgl 3 -basic luciferase reporter vector  (Promega)
90
Promega inserted into the pgl 3 -basic luciferase reporter vector
Inserted Into The Pgl 3 Basic Luciferase Reporter Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/inserted into the pgl 3 -basic luciferase reporter vector/product/Promega
Average 90 stars, based on 1 article reviews
inserted into the pgl 3 -basic luciferase reporter vector - by Bioz Stars, 2026-04
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pgl-3-basic vector  (Promega)
90
Promega pgl-3-basic vector
Pgl 3 Basic Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pgl-3-basic vector/product/Promega
Average 90 stars, based on 1 article reviews
pgl-3-basic vector - by Bioz Stars, 2026-04
90/100 stars
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luciferase activity assay kit  (Promega)
90
Promega luciferase activity assay kit
The constitutive and HNE-inducible activity of putative EpRE sequences in the 5′-flanking region of human GCLC gene. Oligos containing the corresponding EpRE were cloned into pGL-3 promoter vector and the reporters were transfected into HBE1 cells. After being treated with/without 15 μM HNE for 24h, cells were collected and the <t>luciferase</t> activity was measured. The luciferase activity was normalized with co-transfected β-Gal activity. N=3, * P< 0.05 compared with vehicle vector (v: pGL-3 promoter vector), # P< 0.05 compared with control treatment.
Luciferase Activity Assay Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/luciferase activity assay kit/product/Promega
Average 90 stars, based on 1 article reviews
luciferase activity assay kit - by Bioz Stars, 2026-04
90/100 stars
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β -galactosidase plasmid  (Promega)
90
Promega β -galactosidase plasmid
HepG2 cells were transfected with the cyp2b10 gene PBREM-luciferase reporter construct or cotransfected with the PBREM-luciferase reporter construct and a plasmid encoding the mCAR. In addition, all cells were cotransfected with a plasmid <t>encoding</t> <t>β-galactosidase.</t> Transfected cells were treated with androsten-3α-o1 (AND; 8 μM) to suppress endogenous CAR activation, ORPH (0.1 mM) or TCPOBOP (250 nM), or combinations of these reagents. Results are fold induction of reporter-gene activity in AND-treated cells after correction for pGL3 alone; data derived from three to four separate transfections. Different from AND-treated cells: **P<0.01, *P<0.05.
β Galactosidase Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/β -galactosidase plasmid/product/Promega
Average 90 stars, based on 1 article reviews
β -galactosidase plasmid - by Bioz Stars, 2026-04
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prl-cmv vector  (Promega)
90
Promega prl-cmv vector
HepG2 cells were transfected with the cyp2b10 gene PBREM-luciferase reporter construct or cotransfected with the PBREM-luciferase reporter construct and a plasmid encoding the mCAR. In addition, all cells were cotransfected with a plasmid <t>encoding</t> <t>β-galactosidase.</t> Transfected cells were treated with androsten-3α-o1 (AND; 8 μM) to suppress endogenous CAR activation, ORPH (0.1 mM) or TCPOBOP (250 nM), or combinations of these reagents. Results are fold induction of reporter-gene activity in AND-treated cells after correction for pGL3 alone; data derived from three to four separate transfections. Different from AND-treated cells: **P<0.01, *P<0.05.
Prl Cmv Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/prl-cmv vector/product/Promega
Average 90 stars, based on 1 article reviews
prl-cmv vector - by Bioz Stars, 2026-04
90/100 stars
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galactolight kit  (Tropix Inc)
90
Tropix Inc galactolight kit
HepG2 cells were transfected with the cyp2b10 gene PBREM-luciferase reporter construct or cotransfected with the PBREM-luciferase reporter construct and a plasmid encoding the mCAR. In addition, all cells were cotransfected with a plasmid <t>encoding</t> <t>β-galactosidase.</t> Transfected cells were treated with androsten-3α-o1 (AND; 8 μM) to suppress endogenous CAR activation, ORPH (0.1 mM) or TCPOBOP (250 nM), or combinations of these reagents. Results are fold induction of reporter-gene activity in AND-treated cells after correction for pGL3 alone; data derived from three to four separate transfections. Different from AND-treated cells: **P<0.01, *P<0.05.
Galactolight Kit, supplied by Tropix Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/galactolight kit/product/Tropix Inc
Average 90 stars, based on 1 article reviews
galactolight kit - by Bioz Stars, 2026-04
90/100 stars
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pgem-teasy  (Promega)
90
Promega pgem-teasy
HepG2 cells were transfected with the cyp2b10 gene PBREM-luciferase reporter construct or cotransfected with the PBREM-luciferase reporter construct and a plasmid encoding the mCAR. In addition, all cells were cotransfected with a plasmid <t>encoding</t> <t>β-galactosidase.</t> Transfected cells were treated with androsten-3α-o1 (AND; 8 μM) to suppress endogenous CAR activation, ORPH (0.1 mM) or TCPOBOP (250 nM), or combinations of these reagents. Results are fold induction of reporter-gene activity in AND-treated cells after correction for pGL3 alone; data derived from three to four separate transfections. Different from AND-treated cells: **P<0.01, *P<0.05.
Pgem Teasy, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pgem-teasy/product/Promega
Average 90 stars, based on 1 article reviews
pgem-teasy - by Bioz Stars, 2026-04
90/100 stars
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reporter-lysis buffer  (Promega)
90
Promega reporter-lysis buffer
HepG2 cells were transfected with the cyp2b10 gene PBREM-luciferase reporter construct or cotransfected with the PBREM-luciferase reporter construct and a plasmid encoding the mCAR. In addition, all cells were cotransfected with a plasmid <t>encoding</t> <t>β-galactosidase.</t> Transfected cells were treated with androsten-3α-o1 (AND; 8 μM) to suppress endogenous CAR activation, ORPH (0.1 mM) or TCPOBOP (250 nM), or combinations of these reagents. Results are fold induction of reporter-gene activity in AND-treated cells after correction for pGL3 alone; data derived from three to four separate transfections. Different from AND-treated cells: **P<0.01, *P<0.05.
Reporter Lysis Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/reporter-lysis buffer/product/Promega
Average 90 stars, based on 1 article reviews
reporter-lysis buffer - by Bioz Stars, 2026-04
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standard luciferase dna  (Promega)
90
Promega standard luciferase dna
HepG2 cells were transfected with the cyp2b10 gene PBREM-luciferase reporter construct or cotransfected with the PBREM-luciferase reporter construct and a plasmid encoding the mCAR. In addition, all cells were cotransfected with a plasmid <t>encoding</t> <t>β-galactosidase.</t> Transfected cells were treated with androsten-3α-o1 (AND; 8 μM) to suppress endogenous CAR activation, ORPH (0.1 mM) or TCPOBOP (250 nM), or combinations of these reagents. Results are fold induction of reporter-gene activity in AND-treated cells after correction for pGL3 alone; data derived from three to four separate transfections. Different from AND-treated cells: **P<0.01, *P<0.05.
Standard Luciferase Dna, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/standard luciferase dna/product/Promega
Average 90 stars, based on 1 article reviews
standard luciferase dna - by Bioz Stars, 2026-04
90/100 stars
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5′ promoter region on cat gene fragments and mutants  (MdBio Inc)
90
MdBio Inc 5′ promoter region on cat gene fragments and mutants
HepG2 cells were transfected with the cyp2b10 gene PBREM-luciferase reporter construct or cotransfected with the PBREM-luciferase reporter construct and a plasmid encoding the mCAR. In addition, all cells were cotransfected with a plasmid <t>encoding</t> <t>β-galactosidase.</t> Transfected cells were treated with androsten-3α-o1 (AND; 8 μM) to suppress endogenous CAR activation, ORPH (0.1 mM) or TCPOBOP (250 nM), or combinations of these reagents. Results are fold induction of reporter-gene activity in AND-treated cells after correction for pGL3 alone; data derived from three to four separate transfections. Different from AND-treated cells: **P<0.01, *P<0.05.
5′ Promoter Region On Cat Gene Fragments And Mutants, supplied by MdBio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/5′ promoter region on cat gene fragments and mutants/product/MdBio Inc
Average 90 stars, based on 1 article reviews
5′ promoter region on cat gene fragments and mutants - by Bioz Stars, 2026-04
90/100 stars
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prl-tk  (Promega)
90
Promega prl-tk
HepG2 cells were transfected with the cyp2b10 gene PBREM-luciferase reporter construct or cotransfected with the PBREM-luciferase reporter construct and a plasmid encoding the mCAR. In addition, all cells were cotransfected with a plasmid <t>encoding</t> <t>β-galactosidase.</t> Transfected cells were treated with androsten-3α-o1 (AND; 8 μM) to suppress endogenous CAR activation, ORPH (0.1 mM) or TCPOBOP (250 nM), or combinations of these reagents. Results are fold induction of reporter-gene activity in AND-treated cells after correction for pGL3 alone; data derived from three to four separate transfections. Different from AND-treated cells: **P<0.01, *P<0.05.
Prl Tk, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/prl-tk/product/Promega
Average 90 stars, based on 1 article reviews
prl-tk - by Bioz Stars, 2026-04
90/100 stars
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Image Search Results


The constitutive and HNE-inducible activity of putative EpRE sequences in the 5′-flanking region of human GCLC gene. Oligos containing the corresponding EpRE were cloned into pGL-3 promoter vector and the reporters were transfected into HBE1 cells. After being treated with/without 15 μM HNE for 24h, cells were collected and the luciferase activity was measured. The luciferase activity was normalized with co-transfected β-Gal activity. N=3, * P< 0.05 compared with vehicle vector (v: pGL-3 promoter vector), # P< 0.05 compared with control treatment.

Journal:

Article Title: Reexamination of the electrophile response element sequences and context reveals a lack of consensus in gene function

doi: 10.1016/j.bbagrm.2010.05.003

Figure Lengend Snippet: The constitutive and HNE-inducible activity of putative EpRE sequences in the 5′-flanking region of human GCLC gene. Oligos containing the corresponding EpRE were cloned into pGL-3 promoter vector and the reporters were transfected into HBE1 cells. After being treated with/without 15 μM HNE for 24h, cells were collected and the luciferase activity was measured. The luciferase activity was normalized with co-transfected β-Gal activity. N=3, * P< 0.05 compared with vehicle vector (v: pGL-3 promoter vector), # P< 0.05 compared with control treatment.

Article Snippet: Luciferase activity assay kit, pGL-3 bas ic vector, and pGL-3 promoter vector, were from Promega (Madison, WI).

Techniques: Activity Assay, Clone Assay, Plasmid Preparation, Transfection, Luciferase, Control

Effect of EpRE location on EpRE activity. Reporters were constructed as described in the Experimental Procedurals. The reporters were transfected into HBE 1 cells and the luciferase activity was measured. The relative luciferase activity was calculated by using the vehicle vector (pGL-3 basic vector) without HNE exposure as the control. N=3, *, P< 0.05 compared with corresponding reporter without HNE exposure; #, P< 0.05 compared with GCLC-Luc without HNE exposure. GCLC-Luc, −3802/+85 GCLC-luc; m4 GCLC-Luc, −3802/+85 GCLC-Luc with EpRE 4 mutated; in EpRE 4/n1-Luc, EpRE 4/n6-Luc, EpRE 4 was relocated to the position of EpRE n1, and n6 of m4 GCLC-Luc, respectively; in EpRE n1/E4-Luc, EpRE n6/E4-Luc and EpRE 3/E4-Luc, EpRE 4 in GCLC-Luc was replaced with EpRE n1, n6, and EpRE 3, respectively.

Journal:

Article Title: Reexamination of the electrophile response element sequences and context reveals a lack of consensus in gene function

doi: 10.1016/j.bbagrm.2010.05.003

Figure Lengend Snippet: Effect of EpRE location on EpRE activity. Reporters were constructed as described in the Experimental Procedurals. The reporters were transfected into HBE 1 cells and the luciferase activity was measured. The relative luciferase activity was calculated by using the vehicle vector (pGL-3 basic vector) without HNE exposure as the control. N=3, *, P< 0.05 compared with corresponding reporter without HNE exposure; #, P< 0.05 compared with GCLC-Luc without HNE exposure. GCLC-Luc, −3802/+85 GCLC-luc; m4 GCLC-Luc, −3802/+85 GCLC-Luc with EpRE 4 mutated; in EpRE 4/n1-Luc, EpRE 4/n6-Luc, EpRE 4 was relocated to the position of EpRE n1, and n6 of m4 GCLC-Luc, respectively; in EpRE n1/E4-Luc, EpRE n6/E4-Luc and EpRE 3/E4-Luc, EpRE 4 in GCLC-Luc was replaced with EpRE n1, n6, and EpRE 3, respectively.

Article Snippet: Luciferase activity assay kit, pGL-3 bas ic vector, and pGL-3 promoter vector, were from Promega (Madison, WI).

Techniques: Activity Assay, Construct, Transfection, Luciferase, Plasmid Preparation, Control

HepG2 cells were transfected with the cyp2b10 gene PBREM-luciferase reporter construct or cotransfected with the PBREM-luciferase reporter construct and a plasmid encoding the mCAR. In addition, all cells were cotransfected with a plasmid encoding β-galactosidase. Transfected cells were treated with androsten-3α-o1 (AND; 8 μM) to suppress endogenous CAR activation, ORPH (0.1 mM) or TCPOBOP (250 nM), or combinations of these reagents. Results are fold induction of reporter-gene activity in AND-treated cells after correction for pGL3 alone; data derived from three to four separate transfections. Different from AND-treated cells: **P<0.01, *P<0.05.

Journal:

Article Title: Upregulation of cytochromes P 450 2B in rat liver by orphenadrine

doi: 10.1038/sj.bjp.0705305

Figure Lengend Snippet: HepG2 cells were transfected with the cyp2b10 gene PBREM-luciferase reporter construct or cotransfected with the PBREM-luciferase reporter construct and a plasmid encoding the mCAR. In addition, all cells were cotransfected with a plasmid encoding β-galactosidase. Transfected cells were treated with androsten-3α-o1 (AND; 8 μM) to suppress endogenous CAR activation, ORPH (0.1 mM) or TCPOBOP (250 nM), or combinations of these reagents. Results are fold induction of reporter-gene activity in AND-treated cells after correction for pGL3 alone; data derived from three to four separate transfections. Different from AND-treated cells: **P<0.01, *P<0.05.

Article Snippet: The β -galactosidase plasmid, reagents for luciferase assay and pGL-3 basic vector were from Promega.

Techniques: Transfection, Luciferase, Construct, Plasmid Preparation, Activation Assay, Activity Assay, Derivative Assay